Human MYL2(Myosin Light Chain 2, Regulatory, Cardiac) ELISA Kit

Mfr.No.

ELK2290

Alternative Names

CMH10; MLC2; Myosin,Light Chain 2,Regulatory,Cardiac,Slow; Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Reactivity

Human

Detection Range

7.82-500 pg/mL

Sample Type

Tissue homogenates, Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant MYL2 and the recovery rates were calculated by comparing the measured value to the expected amount of MYL2 in samples.

Matrix	Recovery range	Average
serum(n=5)	88-103%	95%
EDTA plasma(n=5)	92-106%	99%
Heparin plasma(n=5)	77-95%	86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MYL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	90-99%	83-97%	81-103%	89-101%
EDTA plasma(n=5)	85-97%	87-96%	82-95%	92-105%
Heparin plasma(n=5)	85-97%	90-99%	82-101%	93-105%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
500.00	1.974	1.878
250.00	1.579	1.483
125.00	1.133	1.037
62.50	0.877	0.781
31.25	0.509	0.413
15.63	0.357	0.261
7.82	0.221	0.125
0.00	0.096	0.000

Sensitivity

3.1 pg/mL

Limit Of Detection

500 pg/mL

UniProt ID	P10916
Antigen	MYL2
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYL2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Metabolic pathway;Cardiovascular biology;Bone metabolism;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15203529	ELK2290	48T	$320		Add to Cart
BHE15203529	ELK2290	96T	$458		Add to Cart

Human MYL2(Myosin Light Chain 2, Regulatory, Cardiac) ELISA Kit

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