Human myeloid differentiation factor 88, MyD88 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11747h

Alternative Names

myeloid differentiation primary response gene (88), MYD88D, myeloid differentiation primary response gene 88

Reactivity

Human

Detection Range

0.312ng/mL-20ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse INSL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	98	93-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse INSL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	90-96
1:200	Average %	100
	Range %	97-103
1:400	Average %	88
	Range %	85-93
1:800	Average %	107
	Range %	103-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q99836
Antigen	myeloid differentiation primary response gene (88)

Applications

ELISA

Research Area

MYD88

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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