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Home / ELISA Kit

Human Myelin protein zero-like protein 1 (MPZL1) ELISA Kit

SKU : BHE13703762

MPZL1 (Myelin Protein Zero Like 1) is a Protein Coding gene. Diseases associated with MPZL1 include Noonan Syndrome 1. Among its related pathways are Adhesion and Tyrosine Kinases / Adaptors. GO annotations related to this gene include structural molecule activity. An important paralog of this gene is MPZ. Cell surface receptor, which is involved in signal transduction processes. Recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. Is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 3 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2 and isoform 3, may be involved in regulation of integrin-mediated cell motility.

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Mfr.No.	KTE61547
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Myelin protein zero-like protein 1 (MPZL1) ELISA Kit employs a two-site sandwich ELISA to quantitate MPZL1.
Alternative Names	MPZL1; FLJ21047; MPZL1b; PZR; PZR1b; PZRa; PZRb; immunoglobulin family transmembrane protein; protein zero related
Detection Method	Colorimetric
Kit Components	Human Myelin protein zero-like protein 1 microplate Human Myelin protein zero-like protein 1 standard Human Myelin protein zero-like protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Myelin protein zero-like protein 1 (MPZL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPZL1. No significant cross-reactivity or interference between Human MPZL1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MPZL1 (Myelin Protein Zero Like 1) is a Protein Coding gene. Diseases associated with MPZL1 include Noonan Syndrome 1. Among its related pathways are Adhesion and Tyrosine Kinases / Adaptors. GO annotations related to this gene include structural molecule activity. An important paralog of this gene is MPZ. Cell surface receptor, which is involved in signal transduction processes. Recruits PTPN11/SHP-2 to the cell membrane and is a putative substrate of PTPN11/SHP-2. Is a major receptor for concanavalin-A (ConA) and is involved in cellular signaling induced by ConA, which probably includes Src family tyrosine-protein kinases. Isoform 3 seems to have a dominant negative role; it blocks tyrosine phosphorylation of MPZL1 induced by ConA. Isoform 1, but not isoform 2 and isoform 3, may be involved in regulation of integrin-mediated cell motility.
Notes	This Human Myelin protein zero-like protein 1 (MPZL1) ELISA Kit employs a two-site sandwich ELISA to quantitate MPZL1 in samples. An antibody specific for MPZL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPZL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPZL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPZL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9019

Applications	ELISA
Applications Notes	This Human Myelin protein zero-like protein 1 (MPZL1) ELISA Kit employs a two-site sandwich ELISA to quantitate MPZL1 in samples. An antibody specific for MPZL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPZL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPZL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPZL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703762	KTE61547	48 T	$339		Add to Cart
BHE13703762	KTE61547	96 T	$589		Add to Cart
BHE13703762	KTE61547	96 Tx5	$2629		Add to Cart
BHE13703762	KTE61547	96 Tx50	$24569		Add to Cart

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