Human mycoplasma pneumoniae (MP) antibody (IgM) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ028034HU

Alternative Names

mycoplasma pneumoniae (MP) antibody (IgM)

Reactivity

Human

Detection Range

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Sample Type

Serum

Assay Type

Indirect

Assay Duration

1-5h

Recovery

The recovery of human S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-105
EDTA plasma (n=4)	100	95-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-96
1:2	Average %	93
	Range %	82-101
1:4	Average %	97
	Range %	93-104
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

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Limit Of Detection

450 nm

Antigen	mycoplasma pneumoniae (MP) antibody (IgM)

Applications

ELISA

Research Area

IgM

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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