Matrices listed below were spiked with certain level of MUSKab and the recovery rates were calculated by comparing the measured value to the expected amount of MUSKab in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
96
EDTA plasma(n=5)
89-103
97
heparin plasma(n=5)
87-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MUSKab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-103%
94-103%
85-104%
EDTA plasma(n=5)
96-101%
88-101%
82-87%
heparin plasma(n=5)
82-98%
81-99%
82-100%
Sensitivity
< 1.406ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
MUSKab ELISA Kit allows for the in vitro quantitative determination of MUSKab concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human MUSKab (Muscle Skeletal Receptor Tyrosine Kinase antibody) ELISA Kit