Human Muscarinic Acetylcholine Receptor M3

Mfr.No.

E1298Hu

Sensitivity

7.18ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

EGBRS;HM3;M-AChR M3;Muscarinic acetylcholine receptor M3;PBS

Molecular Weight

66128

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1298Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	559.05	25.24	4.52
Intra-Assay	2	18	300.2	16.21	5.4
Intra-Assay	3	18	141.11	7.56	5.36

Background

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Protocol

These standard curves of E1298Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.126	2.179	2.121
	2.233
800ng/L	1.719	1.761	1.703
	1.804
400ng/L	1.143	1.218	1.159
	1.293
200ng/L	0.741	0.736	0.677
	0.73
100ng/L	0.387	0.385	0.326
	0.383
0ng/L	0.054	0.058	0
	0.063

Gene ID	1131
UniProt ID	P20309

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102943	E1298Hu	48T	$320		Add to Cart
BHE12102943	E1298Hu	96T	$458		Add to Cart

Human Muscarinic Acetylcholine Receptor M3 - CHRM3 ELISA Kit

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