Human Muscarinic Acetylcholine Receptor M2

Mfr.No.

E2072Hu

Description

This sandwich kit is for the accurate quantitative detection of human Muscarinic Acetylcholine Receptor M2 (also known as MAChRM2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CHRM 2;CHRM2;Muscarinic acetylcholine receptor M2

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

51715

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.19ng/L

Background

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium Channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol.

Gene ID	1129
UniProt ID	P08172
Antigen	CHRM2

Research Area

Others

Protocol

These standard curves of E2072Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.05	2.204	2.151
	2.357
1200ng/L	1.146	1.213	1.16
	1.28
600ng/L	0.674	0.67	0.617
	0.666
300ng/L	0.487	0.437	0.384
	0.387
150ng/L	0.233	0.216	0.163
	0.2
0ng/L	0.057	0.053	0
	0.049

Precision

We measured random samples of E2072Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	640.3	38.31	6
Intra-Assay	2	18	1842.7	52.11	2.8
Intra-Assay	3	18	913.6	48.34	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103624	E2072Hu	48T	$320		Add to Cart
BHE12103624	E2072Hu	96T	$458		Add to Cart

Human Muscarinic Acetylcholine Receptor M2 - M-ACHR M2 ELISA Kit

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