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Home / ELISA Kit

Human Mucin3 (MUC3) ELISA Kit

SKU : BHE13703676

Mucin3 encoded by MUC3 is a membrane-bound mucin that provides protection to gut epithelial cells. Mucin3 contains about 60 tandem repeats, with each repeat being around 60 aa. N-glycosylation enables the Mucin3 to localize on the cell surface, while the C-terminus interacts with the scaffold protein PDZ domain containing 1 (PDZK1). Two transcript variants, one protein-coding and the other non-protein coding, have been found for MUC3.

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Mfr.No.	KTE61461
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mucin3 (MUC3) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC17.
Alternative Names	MUC17; MUC3; membrane mucin MUC17; mucin 17; secreted mucin MUC17; small intestinal mucin MUC3
Detection Method	Colorimetric
Kit Components	Human Mucin3 microplate Human Mucin3 standard Human Mucin3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mucin3 (MUC3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUC17. No significant cross-reactivity or interference between Human MUC17 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Mucin3 encoded by MUC3 is a membrane-bound mucin that provides protection to gut epithelial cells. Mucin3 contains about 60 tandem repeats, with each repeat being around 60 aa. N-glycosylation enables the Mucin3 to localize on the cell surface, while the C-terminus interacts with the scaffold protein PDZ domain containing 1 (PDZK1). Two transcript variants, one protein-coding and the other non-protein coding, have been found for MUC3.
Notes	This Human Mucin3 (MUC3) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC17 in samples. An antibody specific for MUC17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	140453

Applications	ELISA
Applications Notes	This Human Mucin3 (MUC3) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC17 in samples. An antibody specific for MUC17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703676	KTE61461	48 T	$339		Add to Cart
BHE13703676	KTE61461	96 T	$589		Add to Cart
BHE13703676	KTE61461	96 Tx5	$2629		Add to Cart
BHE13703676	KTE61461	96 Tx50	$24569		Add to Cart

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