Human mucin-5 subtype B,MUC5B ELISA kit

Human mucin-5 subtype B,MUC5B ELISA kit

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Mfr.No.

CSB-E11201h

Sensitivity

1.95 ng/mL

Detection Range

7.8 ng/mL-500 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

mucin 5B, oligomeric mucus/gel-forming, MG1, MUC5, MUC9, cervical mucin MUC5B, high molecular weight mucin, mucin 5, subtype B, tracheobronchial, mucin MUC5B, mucin, salivary (high molecular weight)

Recovery

The recovery of rat INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	90
	Range %	85-94
1:200	Average %	95
	Range %	91-99
1:400	Average %	84
	Range %	80-89
1:800	Average %	89
	Range %	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nIU/ml	OD1	OD2	Average	Corrected
1000	2.372	2.234	2.303	2.150
500	1.859	1.812	1.836	1.683
250	1.429	1.365	1.397	1.244
125	1.027	1.097	1.062	0.909
62.5	0.576	0.589	0.583	0.430
31.2	0.341	0.317	0.329	0.176
15.6	0.213	0.207	0.210	0.057
0	0.155	0.151	0.153	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9HC84
Tissue Specificity	Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.

Applications	ELISA
Research Area	MUC5B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503407	CSB-E11201h	96T	$695	Add to Cart
BHE10503407	CSB-E11201h	5x96T	$3336	Add to Cart
BHE10503407	CSB-E11201h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.