Matrices listed below were spiked with certain level of MUC20 and the recovery rates were calculated by comparing the measured value to the expected amount of MUC20 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
96
EDTA plasma(n=5)
93-103
98
heparin plasma(n=5)
87-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MUC20 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
MUC20 ELISA Kit allows for the in vitro quantitative determination of MUC20 concentrations in serum, plasma, tissue homogenates and other biological fluids.