Human MTR(5-Methyltetrahydrofolate Homocysteine Methyltransferase) ELISA Kit

Mfr.No.

ELK4490

Alternative Names

CblG; MS; MeSe; MetH Methionine Synthase; Vitamin-B12 dependent methionine synthase

Reactivity

Human

Detection Range

0.63-40 ng/mL

Sample Type

Tissue homogenates, Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant MTR and the recovery rates were calculated by comparing the measured value to the expected amount of MTR in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	92-107%	99%
Heparin plasma(n=5)	93-107%	100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MTR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	87-101%	82-95%	82-93%
EDTA plasma(n=5)	85-98%	88-104%	86-102%	79-93%
Heparin plasma(n=5)	89-97%	85-94%	87-101%	85-96%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
40.00	2.035	1.949
20.00	1.598	1.512
10.00	1.185	1.099
5.00	0.797	0.711
2.50	0.492	0.406
1.25	0.339	0.253
0.63	0.216	0.130
0.00	0.086	0.000

Sensitivity

0.278 ng/mL

Limit Of Detection

40 ng/mL

UniProt ID	Q99707
Antigen	MTR
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MTR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MTR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MTR in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Enzyme & Kinase;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15202054	ELK4490	48T	$320		Add to Cart
BHE15202054	ELK4490	96T	$458		Add to Cart

Human MTR(5-Methyltetrahydrofolate Homocysteine Methyltransferase) ELISA Kit

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