Human MT-RNR2 (Putative humanin peptide) ELISA kit

Mfr.No.

ELK8996

Alternative Names

MT-RNR2; HN ; Humanin ; Humanin mitochondrial ; HNM

Reactivity

Human

Detection Range

31.25-2000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant MT-RNR2 and the recovery rates were calculated by comparing the measured value to the expected amount of MT-RNR2 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	92-105%	98%
Heparin plasma(n=5)	84-96%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MT-RNR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-94%	79-96%	92-105%	91-99%
EDTA plasma(n=5)	81-93%	78-91%	87-98%	82-95%
Heparin plasma(n=5)	88-102%	84-97%	89-107%	85-92%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.172	2.080
1000.00	1.687	1.595
500.00	1.198	1.106
250.00	0.897	0.805
125.00	0.583	0.491
62.50	0.361	0.269
31.25	0.226	0.134
0.00	0.092	0.000

Sensitivity

12.3 pg/mL

Limit Of Detection

2000 pg/mL

Antigen	MT-RNR2
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MT-RNR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MT-RNR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MT-RNR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MT-RNR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Others
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200829	ELK8996	48T	$320		Add to Cart
BHE15200829	ELK8996	96T	$458		Add to Cart

Human MT-RNR2 (Putative humanin peptide) ELISA kit

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