Matrices listed below were spiked with certain level of MOTS-c and the recovery rates were calculated by comparing the measured value to the expected amount of MOTS-c in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-97
90
EDTA plasma(n=5)
89-99
94
heparin plasma(n=5)
90-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MOTS-c and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
85-104%
89-104%
EDTA plasma(n=5)
89-98%
86-101%
87-97%
heparin plasma(n=5)
88-100%
85-96%
95-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
A0A0C5B5G6
Applications
ELISA
Applications Notes
MOTS-cElisa kit allows for the in vitro quantitative determination of MOTS-c concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human MOTS-c(Mitochondrial-derived peptide MOTS-c)Elisa kit