Human Motilin,MTL ELISA Kit

Human Motilin,MTL ELISA Kit

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Mfr.No.

CSB-E08207h

Alternative Names

Motilin,MTL

Reactivity

Human

Detection Range

18.75 pg/mL-1200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of fish INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-98
EDTA plasma (n=4)	92	90-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-100
1:2	Average %	98
	Range %	92-103
1:4	Average %	85
	Range %	80-89
1:8	Average %	91
	Range %	83-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1600	0.178	0.189	0.184
800	0.322	0.340	0.331
400	0.610	0.634	0.622
200	1.257	1.230	1.244
100	2.335	2.475	2.405

Species

Homo sapiens

Sensitivity

4.68 pg/mL

Limit Of Detection

450 nm

Antigen	Motilin,MTL

Applications

ELISA

Research Area

MTL

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Acute exposure to an electric field induces changes inhuman plasma 9-HODE, 13-HODE, and immunoreactivesubstance P levels: Insight into the molecular mechanismsof electric field therapy Nakagawa-Yagi Y.et al,Integrative Molecular Medicine,/

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SKU	Supplier No.	Size	Your price
BHE10503393	CSB-E08207h	96T	$695	Add to Cart
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BHE10503393	CSB-E08207h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.