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Home / ELISA Kit

Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit

SKU : BHE13702786

SMAD9 or Mothers against decapentaplegic homolog 9 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decapentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGFbeta family members, SMAD9 is involved in cell signalling.
When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor

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Mfr.No.	KTE60570
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD9.
Alternative Names	SMAD9; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B; MAD (mothers against decapentaplegic; Drosophila) homolog 9; MAD; mothers against decapentaplegic homolog 9; Mothers against decapentaplegic; drosophila; homolog o
Detection Method	Colorimetric
Kit Components	Human Mothers against decapentaplegic homolog 9 microplate Human Mothers against decapentaplegic homolog 9 standard Human Mothers against decapentaplegic homolog 9 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMAD9. No significant cross-reactivity or interference between Human SMAD9 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SMAD9 or Mothers against decapentaplegic homolog 9 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decapentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGFbeta family members, SMAD9 is involved in cell signalling. When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor
Notes	This Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD9 in samples. An antibody specific for SMAD9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4093

Applications	ELISA
Applications Notes	This Human Mothers against decapentaplegic homolog 9 (SMAD9) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD9 in samples. An antibody specific for SMAD9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702786	KTE60570	48 T	$339		Add to Cart
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