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Home / ELISA Kit

Human Mothers against decapentaplegic homolog 7 (Smad7) ELISA Kit

SKU : BHE13702787

MAD proteins, originally defined in Drosophila, are essential components of the signaling pathways of the transforming growth factor-beta receptor family. Using a differential display approach in cultured endothelial cells subjected to multiple soluble and biomechanical stimuli, Topper et al. (1997) isolated a human endothelial cell cDNA encoding MADH7, which they called SMAD7.
The predicted 426-amino acid MADH7 protein lacks the C-terminal putative phosphorylation sites present in other MAD proteins, suggesting that it may be distinctly regulated. In situ hybridization and immunohistochemical analyses on human tissues showed that MADH7 is expressed predominantly in vascular endothelium.

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Mfr.No.	KTE60571
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mothers against decapentaplegic homolog 7 (Smad7) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD7.
Alternative Names	SMAD7; CRCS3; FLJ16482; MADH7; MADH8; MAD (mothers against decapentaplegic; Drosophila) homolog 7; MAD; mothers against decapentaplegic homolog 7; Mothers against decapentaplegic; drosophila; homolog of; 7; S
Detection Method	Colorimetric
Kit Components	Human Mothers against decapentaplegic homolog 7 microplate Human Mothers against decapentaplegic homolog 7 standard Human Mothers against decapentaplegic homolog 7 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mothers against decapentaplegic homolog 7 (Smad7) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMAD7. No significant cross-reactivity or interference between Human SMAD7 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MAD proteins, originally defined in Drosophila, are essential components of the signaling pathways of the transforming growth factor-beta receptor family. Using a differential display approach in cultured endothelial cells subjected to multiple soluble and biomechanical stimuli, Topper et al. (1997) isolated a human endothelial cell cDNA encoding MADH7, which they called SMAD7. The predicted 426-amino acid MADH7 protein lacks the C-terminal putative phosphorylation sites present in other MAD proteins, suggesting that it may be distinctly regulated. In situ hybridization and immunohistochemical analyses on human tissues showed that MADH7 is expressed predominantly in vascular endothelium.
Notes	This Human Mothers against decapentaplegic homolog 7 (Smad7) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD7 in samples. An antibody specific for SMAD7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4092

Applications	ELISA
Applications Notes	This Human Mothers against decapentaplegic homolog 7 (Smad7) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD7 in samples. An antibody specific for SMAD7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702787	KTE60571	48 T	$339		Add to Cart
BHE13702787	KTE60571	96 T	$589		Add to Cart
BHE13702787	KTE60571	96 Tx5	$2629		Add to Cart
BHE13702787	KTE60571	96 Tx50	$24569		Add to Cart

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