Human Mothers Against Decapentaplegic Homolog 6

Mfr.No.

E4442Hu

Description

This sandwich kit is for the accurate quantitative detection of Mothers Against Decapentaplegic Homolog 6 (also known as Smad6) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

hSMAD6;MAD homolog 6;Mothers against decapentaplegic homolog 6;Mothers against DPP homolog 6;SMAD 6;SMAD family member 6;Smad6

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

53497

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.72ng/L

Background

Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions.

Gene ID	4091
UniProt ID	O43541
Antigen	SMAD6

Research Area

Others

Protocol

These standard curves of E4442Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.514	2.459	2.412
	2.405
600ng/L	1.441	1.42	1.373
	1.4
300ng/L	0.871	0.803	0.756
	0.735
150ng/L	0.409	0.441	0.393
	0.472
75ng/L	0.231	0.219	0.172
	0.207
0ng/L	0.05	0.047	0
	0.045

Precision

We measured random samples of E4442Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	119.3	6.53	5.5
Intra-Assay	2	18	608.8	17.06	2.8
Intra-Assay	3	18	359.9	20.13	5.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105788	E4442Hu	48T	$320		Add to Cart
BHE12105788	E4442Hu	96T	$458		Add to Cart

Human Mothers Against Decapentaplegic Homolog 6 - SMAD6 ELISA Kit

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