Human Mothers Against Decapentaplegic Homolog 1

Mfr.No.

E0622Hu

Sensitivity

0.25ng/ml

Detection Range

0.5-150ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Mothers Against Decapentaplegic Homolog 1 (also known as Smad1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BSP-1;hSMAD1;JV4-1;MAD homolog 1;Mad-related protein 1;Mothers against decapentaplegic homolog 1;Mothers against DPP homolog 1;SMAD 1;SMAD family member 1;SMAD1;Transforming growth factor-beta-signaling protein 1

Molecular Weight

52260

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0622Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	46.4	1.49	3.2
Intra-Assay	2	18	28.1	1.38	4.9
Intra-Assay	3	18	17.1	1.01	5.9

Background

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

Protocol

These standard curves of E0622Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80ng/ml	2.346	2.25	2.221
	2.155
40ng/ml	1.361	1.391	1.362
	1.421
20ng/ml	0.758	0.695	0.665
	0.632
10ng/ml	0.343	0.369	0.34
	0.395
5ng/ml	0.179	0.148	0.118
	0.117
0ng/ml	0.035	0.03	0
	0.024

Gene ID	4086
UniProt ID	Q15797

Research Area	Signal Transduction; Epigenetics and Nuclear Signaling; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102305	E0622Hu	96T	$458		Add to Cart
BHE12102305	E0622Hu	48T	$320		Add to Cart

Human Mothers Against Decapentaplegic Homolog 1 - SMAD1 ELISA Kit

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