Human Monocyte Chemotactic Protein 4

Mfr.No.

E0068Hu

Description

This sandwich kit is for the accurate quantitative detection of human Monocyte Chemotactic Protein 4 (also known as MCP-4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

C-C motif chemokine 13;C-C motif chemokine 13, long chain;C-C motif chemokine 13, medium chain;C-C motif chemokine 13, short chain;CCL 13;CCL13;CK-beta-10;MCP-4;Monocyte chemoattractant protein 4;Monocyte chemotactic protein 4;NCC-1;Small-inducible cytokine A13

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

10986

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.57ng/L

Background

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Gene ID	6357
UniProt ID	Q99616
Antigen	CCL13

Research Area

Immunology

Protocol

These standard curves of E0068Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.287	2.235	2.188
	2.182
1200ng/L	1.399	1.416	1.369
	1.433
600ng/L	0.948	0.936	0.889
	0.923
300ng/L	0.445	0.468	0.422
	0.492
150ng/L	0.28	0.251	0.204
	0.222
0ng/L	0.046	0.047	0
	0.047

Precision

We measured random samples of E0068Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1037.4	40.18	3.9
Intra-Assay	2	18	736.2	44.43	6
Intra-Assay	3	18	290.4	15.2	5.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101803	E0068Hu	96T	$458		Add to Cart
BHE12101803	E0068Hu	48T	$320		Add to Cart

Human Monocyte Chemotactic Protein 4 - MCP-4 ELISA Kit

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