Human Monocyte Chemotactic Protein 2

Mfr.No.

E0125Hu

Sensitivity

9.44ng/L

Detection Range

20-3800ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Monocyte Chemotactic Protein 2 (also known as MCP-2) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

C-C motif chemokine 8;CCL 8;CCL8;HC14;MCP-2;MCP-2(6-76;Monocyte chemoattractant protein 2;Monocyte chemotactic protein 2;Small-inducible cytokine A8;

Molecular Weight

11246

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0125Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1110.3	49.82	4.5
Intra-Assay	2	18	1506.4	36.41	2.4
Intra-Assay	3	18	670.6	32.21	4.8

Background

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.

Protocol

These standard curves of E0125Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	1.79	1.902	1.863
	2.014
1000ng/L	0.872	0.937	0.898
	1.003
500ng/L	0.577	0.606	0.567
	0.636
250ng/L	0.139	0.194	0.155
	0.25
125ng/L	0.073	0.091	0.052
	0.11
0ng/L	0.035	0.039	0
	0.044

Gene ID	6355
UniProt ID	P80075

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101860	E0125Hu	96T	$458		Add to Cart
BHE12101860	E0125Hu	48T	$320		Add to Cart

Human Monocyte Chemotactic Protein 2 - MCP-2 ELISA Kit

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