Human monocyte chemotactic protein 2,MCP-2 ELISA kit

Human monocyte chemotactic protein 2,MCP-2 ELISA kit

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Mfr.No.

CSB-E04656h

Sensitivity

3.9 pg/ml

Detection Range

15.6 pg/ml - 1000 pg/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 8, HC14, MCP-2, MCP2, SCYA10, SCYA8, monocyte chemoattractant protein 2, monocyte chemotactic protein 2, small inducible cytokine A8, small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte che

Recovery

The recovery of dog C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	90	87-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-105
1:400	Average %	96
	Range %	92-99
1:800	Average %	103
	Range %	98-109
1:1600	Average %	87
	Range %	82-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2000	0.080	0.079	0.080
1000	0.117	0.125	0.121
500	0.202	0.198	0.200
250	0.311	0.305	0.308
125	0.499	0.513	0.506
62.5	0.927	0.933	0.930
31.25	1.457	1.342	1.400
0	2.559	2.438	2.499

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P80075

Applications	ELISA
Research Area	CCL8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503384	CSB-E04656h	96T	$395	Add to Cart
BHE10503384	CSB-E04656h	5x96T	$1896	Add to Cart
BHE10503384	CSB-E04656h	10x96T	$3752.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.