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Home / ELISA Kit

Human Monocarboxylate transporter 1 (SLC16A1) ELISA Kit

SKU : BHE13702842

SLC16A1 mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT. The corresponding cDNA was isolated by an expression cloning strategy and found to encode a protein with 12 putative membrane-spanning regions. The cloned mutant 'mevalonate transporter' differed from its wildtype progenitor by 1 amino acid in the tenth membrane-spanning region, which changed a phenylalanine (wildtype) to a cysteine (mutant). The finding that the wildtype cDNA did not elicit increased mevalonate transport in transfected cells suggested that the wildtype protein is a transporter for a molecule other than mevalonate.

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Mfr.No.	KTE60626
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Monocarboxylate transporter 1 (SLC16A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC16A1.
Alternative Names	SLC16A1; FLJ36745; HHF7; MCT; MCT1; MGC44475; monocarboxylate transporter 1; solute carrier family 16 (monocarboxylic acid transporters); member 1; solute carrier family 16; member 1
Detection Method	Colorimetric
Kit Components	Human Monocarboxylate transporter 1 microplate Human Monocarboxylate transporter 1 standard Human Monocarboxylate transporter 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Monocarboxylate transporter 1 (SLC16A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC16A1. No significant cross-reactivity or interference between Human SLC16A1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SLC16A1 mediates the movement of lactate and pyruvate across cell membranes Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT. The corresponding cDNA was isolated by an expression cloning strategy and found to encode a protein with 12 putative membrane-spanning regions. The cloned mutant 'mevalonate transporter' differed from its wildtype progenitor by 1 amino acid in the tenth membrane-spanning region, which changed a phenylalanine (wildtype) to a cysteine (mutant). The finding that the wildtype cDNA did not elicit increased mevalonate transport in transfected cells suggested that the wildtype protein is a transporter for a molecule other than mevalonate.
Notes	This Human Monocarboxylate transporter 1 (SLC16A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC16A1 in samples. An antibody specific for SLC16A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC16A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC16A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC16A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6566

Applications	ELISA
Applications Notes	This Human Monocarboxylate transporter 1 (SLC16A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC16A1 in samples. An antibody specific for SLC16A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC16A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC16A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC16A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702842	KTE60626	48 T	$339		Add to Cart
BHE13702842	KTE60626	96 T	$589		Add to Cart
BHE13702842	KTE60626	96 Tx5	$2629		Add to Cart
BHE13702842	KTE60626	96 Tx50	$24569		Add to Cart

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