Human Mitogen-activated Protein Kinase 6

Mfr.No.

E0743Hu

Alternative Names

ERK-3;Extracellular signal-regulated kinase 3;MAP kinase 6;MAP kinase isoform p97;MAPK 6;MAPK6;Mitogen-activated protein kinase 6;p97-MAPK

Reactivity

Human

Detection Range

0.2-60ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

82681

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.092ng/mL

Background

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).

Gene ID	5597
UniProt ID	Q16659
Antigen	MAPK6

Research Area

Others

Protocol

These standard curves of E0743Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/mL	2.203	2.278	2.22
	2.353
16ng/mL	1.41	1.452	1.394
	1.493
8ng/mL	0.773	0.781	0.723
	0.788
4ng/mL	0.503	0.482	0.424
	0.461
2ng/mL	0.272	0.269	0.212
	0.267
0ng/mL	0.047	0.058	0
	0.068

Precision

We measured random samples of E0743Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.2	0.46	5
Intra-Assay	2	18	16	0.53	3.3
Intra-Assay	3	18	5.7	0.35	6.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102422	E0743Hu	96T	$458		Add to Cart
BHE12102422	E0743Hu	48T	$320		Add to Cart

Human Mitogen-activated Protein Kinase 6 - MAPK6 ELISA Kit

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