Human Mitogen-activated Protein Kinase 4

Mfr.No.

E5689Hu

Sensitivity

7.83ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

FLH21957;HEL-S-31;HGK;HPK/GCK-like kinase HGK;MAPK/ERK kinase 4;MEK kinase 4;MEKKK 4;MEKKK4;Mitogen-activated protein kinase kinase 4;Nck-interacting kinase;NIK

Molecular Weight

142101

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5689Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	262.55	16.29	6.2
Intra-Assay	2	18	674.33	35.89	5.32
Intra-Assay	3	18	907.66	34.35	3.78

Background

MAP4K4, also known as hepatocyte progenitor kinase-like/germinal center kinase-like kinase (HGK) and Nck-interacting kinase (NIK) is an enzyme, specifically a serine/threonine (S/T) kinase encoded by the MAP4K4 gene in humans. MAP4K4 is involved in a wide array of physiological processes including cell migration, proliferation and adhesion; its activity has been implicated in systemic inflammation, metabolic disorders, cardiovascular disease and cancer.

Protocol

These standard curves of E5689Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.178	2.225	2.205
	2.273
800ng/L	1.335	1.325	1.304
	1.314
400ng/L	0.736	0.687	0.666
	0.638
200ng/L	0.371	0.382	0.361
	0.392
100ng/L	0.2	0.183	0.163
	0.166
0ng/L	0.021	0.021	0
	0.02

Gene ID	9448
UniProt ID	O95819

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107051	E5689Hu	48T	$320		Add to Cart
BHE12107051	E5689Hu	96T	$458		Add to Cart

Human Mitogen-activated Protein Kinase 4 - MAP4K4 ELISA Kit

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