Human Mitogen-activated Protein Kinase 4

Mfr.No.

E5689Hu

Alternative Names

FLH21957;HEL-S-31;HGK;HPK/GCK-like kinase HGK;MAPK/ERK kinase 4;MEK kinase 4;MEKKK 4;MEKKK4;Mitogen-activated protein kinase kinase 4;Nck-interacting kinase;NIK

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

142101

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.83ng/L

Background

MAP4K4, also known as hepatocyte progenitor kinase-like/germinal center kinase-like kinase (HGK) and Nck-interacting kinase (NIK) is an enzyme, specifically a serine/threonine (S/T) kinase encoded by the MAP4K4 gene in humans. MAP4K4 is involved in a wide array of physiological processes including cell migration, proliferation and adhesion; its activity has been implicated in systemic inflammation, metabolic disorders, cardiovascular disease and cancer.

Gene ID	9448
UniProt ID	O95819
Antigen	MAP4K4

Research Area

Signal Transduction

Protocol

These standard curves of E5689Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.178	2.225	2.205
	2.273
800ng/L	1.335	1.325	1.304
	1.314
400ng/L	0.736	0.687	0.666
	0.638
200ng/L	0.371	0.382	0.361
	0.392
100ng/L	0.2	0.183	0.163
	0.166
0ng/L	0.021	0.021	0
	0.02

Precision

We measured random samples of E5689Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	262.55	16.29	6.2
Intra-Assay	2	18	674.33	35.89	5.32
Intra-Assay	3	18	907.66	34.35	3.78

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107051	E5689Hu	48T	$320		Add to Cart
BHE12107051	E5689Hu	96T	$458		Add to Cart

Human Mitogen-activated Protein Kinase 4 - MAP4K4 ELISA Kit

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