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Home / ELISA Kit

Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit

SKU : BHE13703949

Mitogen Activated Protein Kinase 13 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.
This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.

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Mfr.No.	KTE61734
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK13.
Alternative Names	MAPK13; MGC99536; PRKM13; SAPK4; p38delta; mitogen-activated protein kinase p38 delta; stress-activated protein kinase 4
Detection Method	Colorimetric
Kit Components	Human Mitogen-activated protein kinase 13 microplate Human Mitogen-activated protein kinase 13 standard Human Mitogen-activated protein kinase 13 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPK13. No significant cross-reactivity or interference between Human MAPK13 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Mitogen Activated Protein Kinase 13 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
Notes	This Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK13 in samples. An antibody specific for MAPK13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5603

Applications	ELISA
Applications Notes	This Human Mitogen-activated protein kinase 13 (MAPK13) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK13 in samples. An antibody specific for MAPK13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703949	KTE61734	48 T	$339		Add to Cart
BHE13703949	KTE61734	96 T	$589		Add to Cart
BHE13703949	KTE61734	96 Tx5	$2629		Add to Cart
BHE13703949	KTE61734	96 Tx50	$24569		Add to Cart

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