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Home / ELISA Kit

Human Mitogen-activated protein kinase 1 (MAPK1) ELISA Kit

SKU : BHE13703951

Mitogen-activated protein kinase 1 also known as MAPK1, p42MAPK, and ERK2is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.
The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

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Mfr.No.	KTE61736
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mitogen-activated protein kinase 1 (MAPK1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK1.
Alternative Names	MAPK1; ERK; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; extracellular signal-regulated kinase 2; extracellular signal-regulated kinase-2; mitogen-activated protein kinase 2; protein tyro
Detection Method	Colorimetric
Kit Components	Human Mitogen-activated protein kinase 1 microplate Human Mitogen-activated protein kinase 1 standard Human Mitogen-activated protein kinase 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mitogen-activated protein kinase 1 (MAPK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPK1. No significant cross-reactivity or interference between Human MAPK1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Mitogen-activated protein kinase 1 also known as MAPK1, p42MAPK, and ERK2is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Notes	This Human Mitogen-activated protein kinase 1 (MAPK1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK1 in samples. An antibody specific for MAPK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5594

Applications	ELISA
Applications Notes	This Human Mitogen-activated protein kinase 1 (MAPK1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK1 in samples. An antibody specific for MAPK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703951	KTE61736	48 T	$339		Add to Cart
BHE13703951	KTE61736	96 T	$589		Add to Cart
BHE13703951	KTE61736	96 Tx5	$2629		Add to Cart
BHE13703951	KTE61736	96 Tx50	$24569		Add to Cart

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