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Home / ELISA Kit

Human Mitochondrial import receptor subunit TOM70 (TOMM70A) ELISA Kit

SKU : BHE13702378

The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria.TOMM70A is 29% identical to the mitochondrial precursor protein import receptor of Neurospora crassa. RT-PCR analysis detected ubiquitous expression of KIAA0719, with relatively weak expression in pancreas, testis, and spleen.the cytosolic chaperones HSP90 and HSP70 dock onto a specialized tetratricopeptide domain in the import receptor TOMM70 at the outer mitochondrial membrane. This interaction served to deliver a set of preproteins to the receptor for subsequent membrane translocation dependent on the HSP90 ATPase. Disruption of the chaperone/TOMM70 recognition inhibited the import of these preproteins into mitochondria.

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Mfr.No.	KTE60159
Description	This Human Mitochondrial import receptor subunit TOM70 (TOMM70A) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM70A.
Alternative Names	TOMM70A; FLJ90470; translocase of outer mitochondrial membrane 70 homolog A
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Mitochondrial import receptor subunit TOM70 microplate Human Mitochondrial import receptor subunit TOM70 standard Human Mitochondrial import receptor subunit TOM70 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mitochondrial import receptor subunit TOM70 (TOMM70A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOMM70A. No significant cross-reactivity or interference between Human TOMM70A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria.TOMM70A is 29% identical to the mitochondrial precursor protein import receptor of Neurospora crassa. RT-PCR analysis detected ubiquitous expression of KIAA0719, with relatively weak expression in pancreas, testis, and spleen.the cytosolic chaperones HSP90 and HSP70 dock onto a specialized tetratricopeptide domain in the import receptor TOMM70 at the outer mitochondrial membrane. This interaction served to deliver a set of preproteins to the receptor for subsequent membrane translocation dependent on the HSP90 ATPase. Disruption of the chaperone/TOMM70 recognition inhibited the import of these preproteins into mitochondria.
Notes	This Human Mitochondrial import receptor subunit TOM70 (TOMM70A) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM70A in samples. An antibody specific for TOMM70A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM70A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM70A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM70A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	9868
Antigen	TOMM70A
Tag	TOMM70A
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Mitochondrial import receptor subunit TOM70 (TOMM70A) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM70A in samples. An antibody specific for TOMM70A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM70A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM70A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM70A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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