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Home / ELISA Kit

Human Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit

SKU : BHE13702383

The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins from the cytosol into the mitochondria. The deduced 142-amino acid human protein, which is approximately 33% similar to the yeast proteins, contains an N-terminal negatively charged region, an internal hydrophobic transmembrane region, and a C-terminal region with a glutamine-rich segment. Immunoblot analysis and fluorescence microscopy showed expression of a 21-kD mitochondrial membrane protein with its N and C termini exposed to the cytosol and intermembrane space of the mitochondrial outer membrane, respectively.An association between the rat Tomm22 protein, which is 94% identical to the human protein, and Tomm40 was observed.

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Mfr.No.	KTE60164
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM22.
Alternative Names	TOMM22; 1C9-2; MST065; MSTP065; TOM22; mitochondrial import receptor Tom22
Detection Method	Colorimetric
Kit Components	Human Mitochondrial import receptor subunit TOM22 homolog microplate Human Mitochondrial import receptor subunit TOM22 homolog standard Human Mitochondrial import receptor subunit TOM22 homolog detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOMM22. No significant cross-reactivity or interference between Human TOMM22 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins from the cytosol into the mitochondria. The deduced 142-amino acid human protein, which is approximately 33% similar to the yeast proteins, contains an N-terminal negatively charged region, an internal hydrophobic transmembrane region, and a C-terminal region with a glutamine-rich segment. Immunoblot analysis and fluorescence microscopy showed expression of a 21-kD mitochondrial membrane protein with its N and C termini exposed to the cytosol and intermembrane space of the mitochondrial outer membrane, respectively.An association between the rat Tomm22 protein, which is 94% identical to the human protein, and Tomm40 was observed.
Notes	This Human Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM22 in samples. An antibody specific for TOMM22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	56993

Applications	ELISA
Applications Notes	This Human Mitochondrial import receptor subunit TOM22 homolog (TOMM22) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM22 in samples. An antibody specific for TOMM22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM22 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
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