Human MIP3b(Macrophage Inflammatory Protein 3 Beta) ELISA Kit

Mfr.No.

ELK2181

Alternative Names

CCL19; CKb11; ELC; MIP3-B; SCYA19; Chemokine C-C-Motif Ligand 19; Beta Chemokine Exodus-3; CK Beta-11; EBI1-Ligand Chemokine; Small Inducible Cytokine Subfamily A19

Reactivity

Human

Detection Range

15.63-1000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant MIP3b and the recovery rates were calculated by comparing the measured value to the expected amount of MIP3b in samples.

Matrix	Recovery range	Average
serum(n=5)	77-95%	86%
EDTA plasma(n=5)	94-106%	100%
Heparin plasma(n=5)	80-97%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP3b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-97%	87-96%	85-96%	85-95%
EDTA plasma(n=5)	86-95%	93-103%	87-98%	85-96%
Heparin plasma(n=5)	92-102%	81-93%	86-98%	91-103%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.266	2.198
500.00	1.631	1.563
250.00	1.135	1.067
125.00	0.847	0.779
62.50	0.508	0.440
31.25	0.357	0.289
15.63	0.225	0.157
0.00	0.068	0.000

Sensitivity

6.4 pg/mL

Limit Of Detection

1000 pg/mL

UniProt ID	Q99731
Antigen	MIP3b
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIP3b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIP3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIP3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIP3b in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Cytokine;Infection immunity;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15203598	ELK2181	48T	$320		Add to Cart
BHE15203598	ELK2181	96T	$458		Add to Cart

Human MIP3b(Macrophage Inflammatory Protein 3 Beta) ELISA Kit

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