Human milk fat globule-EGF factor 8 protein(MFGE8) ELISA kit

Human milk fat globule-EGF factor 8 protein(MFGE8) ELISA kit

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Mfr.No.

CSB-E12637h

Alternative Names

milk fat globule-EGF factor 8 protein, BA46, EDIL1, HsT19888, MFG-E8, OAcGD3S, SED1, SPAG10, hP47, O-acetyl disialoganglioside synthase, lactadherin, medin, sperm associated antigen 10, sperm surface protein hP47

Reactivity

Human

Detection Range

781.25 pg/mL-50000 pg/mL

Sample Type

Serum, Cell culture supernatants, Urine, Cerebrospinal fluid

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CSN1S1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Breast milk(n=4)
1:10	Average %	90
	Range %	86-97
1:20	Average %	93
	Range %	89-99
1:40	Average %	95
	Range %	90-107
1:80	Average %	92
	Range %	87-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
600	0.215	0.233	0.224
300	0.349	0.374	0.362
150	0.655	0.612	0.634
75	1.358	1.326	1.342
37.5	1.764	1.727	1.746
0	2.631	2.419	2.525

Species

Homo sapiens

Sensitivity

195.31 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q08431
Antigen	milk fat globule-EGF factor 8 protein

Applications

ELISA

Research Area

MFGE8

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503361	CSB-E12637h	96T	$790	Add to Cart
BHE10503361	CSB-E12637h	5x96T	$3792	Add to Cart
BHE10503361	CSB-E12637h	10x96T	$7505	Add to Cart

The recovery of human CSN1S1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Breast milk (n=5)