Human milk fat globule-EGF factor 8 protein(MFGE8) ELISA kit

Human milk fat globule-EGF factor 8 protein(MFGE8) ELISA kit

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Mfr.No.

CSB-E12637h

Sensitivity

195.31 pg/mL

Detection Range

781.25 pg/mL-50000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

milk fat globule-EGF factor 8 protein, BA46, EDIL1, HsT19888, MFG-E8, OAcGD3S, SED1, SPAG10, hP47, O-acetyl disialoganglioside synthase, lactadherin, medin, sperm associated antigen 10, sperm surface protein hP47

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CSN1S1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Breast milk(n=4)
1:10	Average %	90
	Range %	86-97
1:20	Average %	93
	Range %	89-99
1:40	Average %	95
	Range %	90-107
1:80	Average %	92
	Range %	87-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
600	0.215	0.233	0.224
300	0.349	0.374	0.362
150	0.655	0.612	0.634
75	1.358	1.326	1.342
37.5	1.764	1.727	1.746
0	2.631	2.419	2.525

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q08431

Applications	ELISA
Research Area	MFGE8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503361	CSB-E12637h	96T	$790	Add to Cart
BHE10503361	CSB-E12637h	5x96T	$3792	Add to Cart
BHE10503361	CSB-E12637h	10x96T	$7505	Add to Cart

The recovery of human CSN1S1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Breast milk (n=5)