Human Microtubule-associated proteins 1A/1B light chain 3B(MAP1LC3B) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL013403HU

Alternative Names

microtubule-associated protein 1 light chain 3 beta, LC3B, MAP1A/1BLC3, microtubule-associated proteins 1A/1B light chain 3

Reactivity

Human

Detection Range

18.75 pg/mL-1200 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Duration

1-5h

Recovery

The recovery of human KLK 6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-93
EDTA plasma (n=4)	96	92-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-95
1:2	Average %	95
	Range %	91-99
1:4	Average %	95
	Range %	88-102
1:8	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

4.67 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9GZQ8
Antigen	microtubule-associated protein 1 light chain 3 beta

Applications

ELISA

Research Area

MAP1LC3B

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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