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Home / ELISA Kit

Human Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2) ELISA Kit

SKU : BHE13703956

MAP1LC3b2 Belongs to the MAP1 LC3 family.MAP1LC3b is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.The deduced 125-amino acid protein shares 94% identity with rat Map1lc3. Northern blot analysis detected transcripts of 2.5 and 1.0 kb abundantly expressed in heart, brain, skeletal muscle, and testis, with weaker expression in all other tissues examined. MAP1LC3B was posttranslationally modified. Unlike MAP1LC3A and MAP1LC3C, however, MAP1LC3B did not undergo C-terminal cleavage and did not require the conserved gly120 for posttranslational modification.

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Mfr.No.	KTE61741
Description	This Human Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1LC3B2.
Alternative Names	MAP1LC3B2; Microtubule-associated proteins 1A/1B light chain 3B-like protein; microtubule-associated protein 1 light chain 3 beta-like
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Microtubule-associated proteins 1A/1B light chain 3 beta 2 microplate Human Microtubule-associated proteins 1A/1B light chain 3 beta 2 standard Human Microtubule-associated proteins 1A/1B light chain 3 beta 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAP1LC3B2. No significant cross-reactivity or interference between Human MAP1LC3B2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MAP1LC3b2 Belongs to the MAP1 LC3 family.MAP1LC3b is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.The deduced 125-amino acid protein shares 94% identity with rat Map1lc3. Northern blot analysis detected transcripts of 2.5 and 1.0 kb abundantly expressed in heart, brain, skeletal muscle, and testis, with weaker expression in all other tissues examined. MAP1LC3B was posttranslationally modified. Unlike MAP1LC3A and MAP1LC3C, however, MAP1LC3B did not undergo C-terminal cleavage and did not require the conserved gly120 for posttranslational modification.
Notes	This Human Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1LC3B2 in samples. An antibody specific for MAP1LC3B2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1LC3B2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1LC3B2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1LC3B2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	643246
Antigen	MAP1LC3B2
Tag	MAP1LC3B2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1LC3B2 in samples. An antibody specific for MAP1LC3B2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1LC3B2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1LC3B2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1LC3B2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13703956	KTE61741	48 T	$339		Add to Cart
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