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Home / ELISA Kit

Human Microtubule-associated protein 1A (MAP1A) ELISA Kit

SKU : BHE13703960

These proteins have been divided into 2 main groups by molecular mass, high molecular weight MAPs, which include MAP1A, MAP1B, and MAP2, and another group of intermediate-sized proteins, which include the abundant tau MAPs. MAP1B, also named MAP5, is a component of long cross-bridges between microtubules and is a filamentous molecule with a small spherical segment at one end.Lien et al. (1994) completely cloned and sequenced the human MAP1B gene. By comparison of human MAP1B with sequence databases, they identified a MAP1B-related gene that is probably the human homolog of rat MAP1A. The human MAP2A gene is expressed at high levels in brain and spinal cord and at much lower levels in muscle.

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Mfr.No.	KTE61745
Description	This Human Microtubule-associated protein 1A (MAP1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1A.
Alternative Names	MAP1A; FLJ77111; MAP1L; MTAP1A; microtubule-associated protein 1-like
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Microtubule-associated protein 1A microplate Human Microtubule-associated protein 1A standard Human Microtubule-associated protein 1A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Microtubule-associated protein 1A (MAP1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAP1A. No significant cross-reactivity or interference between Human MAP1A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	These proteins have been divided into 2 main groups by molecular mass, high molecular weight MAPs, which include MAP1A, MAP1B, and MAP2, and another group of intermediate-sized proteins, which include the abundant tau MAPs. MAP1B, also named MAP5, is a component of long cross-bridges between microtubules and is a filamentous molecule with a small spherical segment at one end.Lien et al. (1994) completely cloned and sequenced the human MAP1B gene. By comparison of human MAP1B with sequence databases, they identified a MAP1B-related gene that is probably the human homolog of rat MAP1A. The human MAP2A gene is expressed at high levels in brain and spinal cord and at much lower levels in muscle.
Notes	This Human Microtubule-associated protein 1A (MAP1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1A in samples. An antibody specific for MAP1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	4130
Antigen	MAP1A
Tag	MAP1A
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Microtubule-associated protein 1A (MAP1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1A in samples. An antibody specific for MAP1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703960	KTE61745	48 T	$339		Add to Cart
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