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Home / ELISA Kit

Human Microcephalin (MCPH1) ELISA Kit

SKU : BHE13703904

MCPH1 encodes a DNA damage response protein. Microcephalin may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in MCPH1 have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described.

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Mfr.No.	KTE61689
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Microcephalin (MCPH1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCPH1.
Alternative Names	MCPH1; BRIT1; FLJ12847; MCT; BRCT-repeat inhibitor of TERT expression 1; microcephalin; microcephaly; primary autosomal recessive 1
Detection Method	Colorimetric
Kit Components	Human Microcephalin microplate Human Microcephalin standard Human Microcephalin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Microcephalin (MCPH1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MCPH1. No significant cross-reactivity or interference between Human MCPH1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MCPH1 encodes a DNA damage response protein. Microcephalin may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in MCPH1 have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described.
Notes	This Human Microcephalin (MCPH1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCPH1 in samples. An antibody specific for MCPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	79648

Applications	ELISA
Applications Notes	This Human Microcephalin (MCPH1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCPH1 in samples. An antibody specific for MCPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703904	KTE61689	48 T	$339		Add to Cart
BHE13703904	KTE61689	96 T	$589		Add to Cart
BHE13703904	KTE61689	96 Tx5	$2629		Add to Cart
BHE13703904	KTE61689	96 Tx50	$24569		Add to Cart

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