Human Mhc Class I Polypeptide-related Sequence B

Mfr.No.

E5670Hu

Alternative Names

MHC class I polypeptide-related sequence B;MICB;MIC-B

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

42575

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.45ng/L

Background

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

Gene ID	4277
UniProt ID	Q29980
Antigen	MICB

Research Area

Immunology

Protocol

These standard curves of E5670Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.416	2.407	2.365
	2.397
1200ng/L	1.468	1.529	1.487
	1.591
600ng/L	0.838	0.78	0.738
	0.722
300ng/L	0.395	0.436	0.394
	0.477
150ng/L	0.251	0.237	0.195
	0.222
0ng/L	0.035	0.042	0
	0.049

Precision

We measured random samples of E5670Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1234.5	44.29	3.6
Intra-Assay	2	18	327.2	18.91	5.8
Intra-Assay	3	18	680.8	29.56	4.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107032	E5670Hu	48T	$320		Add to Cart
BHE12107032	E5670Hu	96T	$458		Add to Cart

Human Mhc Class I Polypeptide-related Sequence B - MICB ELISA Kit

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