Human MHC antibody(anti-myosin heavy chain antibody)ELISA Kit
Alternative Names
MHC antibody ELISA Kit, anti-myosin heavy chain antibody ELISA Kit
Reactivity
Human
Detection Method
HRP Indirect ELISA
Detection Range
0.313-20ng/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of MHC antibody and the recovery rates were calculated by comparing the measured value to the expected amount of MHC antibody in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-96
90
EDTA plasma(n=5)
90-104
99
heparin plasma(n=5)
88-100
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MHC antibody and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
87-105%
88-105%
EDTA plasma(n=5)
84-96%
94-101%
82-94%
heparin plasma(n=5)
80-92%
84-91%
80-95%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
MHC antibodyELISA Kit allows for the in vitro quantitative determination of MHC antibody concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
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