Human Methylenetetrahydrofolate Reductase

Mfr.No.

E4352hu

Sensitivity

0.093ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Methylenetetrahydrofolate Reductase (also known as MTHFR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Methylenetetrahydrofolate reductase;MTHFR

Molecular Weight

74597

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4352hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.3	0.81	4.7
Intra-Assay	2	18	20	0.56	2.8
Intra-Assay	3	18	8.7	0.38	4.3

Background

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine.

Protocol

These standard curves of E4352hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.143	2.073	2.016
	2.003
16ng/ml	1.037	1.102	1.045
	1.167
8ng/ml	0.491	0.525	0.468
	0.559
4ng/ml	0.242	0.234	0.177
	0.226
2ng/ml	0.078	0.099	0.042
	0.12
0ng/ml	0.051	0.057	0
	0.064

Gene ID	4524
UniProt ID	P42898

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105714	E4352hu	48T	$320		Add to Cart
BHE12105714	E4352hu	96T	$458		Add to Cart

Human Methylenetetrahydrofolate Reductase - MTHFR ELISA Kit

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