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Home / ELISA Kit

Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit

SKU : BHE13703912

The MCCD1 gene encodes a deduced 119-amino acid protein containing a putative coiled-coil domain at its C terminus and a predicted mitochondrial localization signal at its N terminus. RT-PCR analysis detected expression of MCCD1 in all human tissues tested, at highest levels in adult and fetal kidney, liver and lung, and fetal brain, and at lower levels in fetal spleen.
The human and pig proteins share 65.9% overall sequence identity but their C-terminal domains are highly conserved, showing 92% identity over 53 residues. No orthologs were identified in any other species, suggesting that the gene arose relatively recently in evolution. Transient expression in mammalian cells of MCCD1 fused at its C terminus to either EGFP or the T7-epitope tag showed that the protein is localized to mitochondria.

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Mfr.No.	KTE61697
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCCC1.
Alternative Names	MCCC1; DKFZp686B20267; FLJ25545; MCC-B; MCCA; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha
Detection Method	Colorimetric
Kit Components	Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial microplate Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial standard Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MCCC1. No significant cross-reactivity or interference between Human MCCC1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The MCCD1 gene encodes a deduced 119-amino acid protein containing a putative coiled-coil domain at its C terminus and a predicted mitochondrial localization signal at its N terminus. RT-PCR analysis detected expression of MCCD1 in all human tissues tested, at highest levels in adult and fetal kidney, liver and lung, and fetal brain, and at lower levels in fetal spleen. The human and pig proteins share 65.9% overall sequence identity but their C-terminal domains are highly conserved, showing 92% identity over 53 residues. No orthologs were identified in any other species, suggesting that the gene arose relatively recently in evolution. Transient expression in mammalian cells of MCCD1 fused at its C terminus to either EGFP or the T7-epitope tag showed that the protein is localized to mitochondria.
Notes	This Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCCC1 in samples. An antibody specific for MCCC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCCC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCCC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCCC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	56922

Applications	ELISA
Applications Notes	This Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCCC1 in samples. An antibody specific for MCCC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCCC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCCC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCCC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703912	KTE61697	48 T	$339		Add to Cart
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