Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial(MCCC1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL013572HU

Reactivity

Human

Detection Range

0.78 ng/ml-50ng/ml

Sample Type

Cell lysates, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	103	100-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	96-102
1:2000	Average %	103
	Range %	100-106
1:4000	Average %	89
	Range %	84-94
1:8000	Average %	96
	Range %	94-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.19 ng/ml

Limit Of Detection

450 nm

UniProt ID	Q96RQ3
Antigen	methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)

Applications

ELISA

Research Area

MCCC1

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10503346	CSB-EL013572HU	96T	$695	Add to Cart
BHE10503346	CSB-EL013572HU	5x96T	$3336	Add to Cart
BHE10503346	CSB-EL013572HU	10x96T	$6602.50	Add to Cart