Human Methylcrotonoyl-CoA Carboxylase Subunit Alpha

Mfr.No.

E5668Hu

Alternative Names

3-methylcrotonyl-CoA carboxylase 1;3-methylcrotonyl-CoA carboxylase biotin-containing subunit;3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha;MCCA;MCCase subunit alpha;MCC-B;MCCC1;MCCCalpha;Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial;

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

80473

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.38ng/L

Background

Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.

Gene ID	56922
UniProt ID	Q96RQ3
Antigen	MCCC1

Research Area

Tags & Cell Markers

Protocol

These standard curves of E5668Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.459	2.569	2.522
	2.678
800ng/L	1.385	1.412	1.366
	1.44
400ng/L	0.847	0.86	0.814
	0.874
200ng/L	0.483	0.469	0.423
	0.455
100ng/L	0.193	0.234	0.188
	0.276
0ng/L	0.054	0.047	0
	0.04

Precision

We measured random samples of E5668Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	176.41	10.05	5.7
Intra-Assay	2	18	955.8	27.47	2.87
Intra-Assay	3	18	460.75	27.13	5.89

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107030	E5668Hu	48T	$320		Add to Cart
BHE12107030	E5668Hu	96T	$458		Add to Cart

Human Methylcrotonoyl-CoA Carboxylase Subunit Alpha - MCCC1 ELISA Kit

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