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Home / ELISA Kit

Human Methionine synthase (MTR) ELISA Kit

SKU : BHE13703693

MTR encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for MTR.

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Mfr.No.	KTE61478
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Methionine synthase (MTR) ELISA Kit employs a two-site sandwich ELISA to quantitate MTR.
Alternative Names	MTR; FLJ33168; FLJ43216; FLJ45386; MS; cblG; 5-methyltetrahydrofolate-homocysteine methyltransferase 1; cobalamin-dependent methionine synthase; methionine synthase; vitamin-B12 dependent methionine synthas
Detection Method	Colorimetric
Kit Components	Human Methionine synthase microplate Human Methionine synthase standard Human Methionine synthase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Methionine synthase (MTR) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTR. No significant cross-reactivity or interference between Human MTR and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MTR encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for MTR.
Notes	This Human Methionine synthase (MTR) ELISA Kit employs a two-site sandwich ELISA to quantitate MTR in samples. An antibody specific for MTR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4552

Applications	ELISA
Applications Notes	This Human Methionine synthase (MTR) ELISA Kit employs a two-site sandwich ELISA to quantitate MTR in samples. An antibody specific for MTR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703693	KTE61478	48 T	$339		Add to Cart
BHE13703693	KTE61478	96 T	$589		Add to Cart
BHE13703693	KTE61478	96 Tx5	$2629		Add to Cart
BHE13703693	KTE61478	96 Tx50	$24569		Add to Cart

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