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Home / ELISA Kit

Human Methionine aminopeptidase 2 (METAP2) ELISA Kit

SKU : BHE13703858

Methionine aminopeptidase 2 encoded by METAP2 is a member of the methionyl aminopeptidase family. Methionine aminopeptidase 2 functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of METAP2 is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of METAP2 is located on chromosome 2. Several transcript variants encoding different isoforms have been found for METAP2.

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Mfr.No.	KTE61643
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Methionine aminopeptidase 2 (METAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate METAP2.
Alternative Names	METAP2; MAP2; MNPEP; p67; p67eIF2; eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; peptidase M 2
Detection Method	Colorimetric
Kit Components	Human Methionine aminopeptidase 2 microplate Human Methionine aminopeptidase 2 standard Human Methionine aminopeptidase 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Methionine aminopeptidase 2 (METAP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human METAP2. No significant cross-reactivity or interference between Human METAP2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Methionine aminopeptidase 2 encoded by METAP2 is a member of the methionyl aminopeptidase family. Methionine aminopeptidase 2 functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of METAP2 is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of METAP2 is located on chromosome 2. Several transcript variants encoding different isoforms have been found for METAP2.
Notes	This Human Methionine aminopeptidase 2 (METAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate METAP2 in samples. An antibody specific for METAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMETAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for METAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of METAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10988

Applications	ELISA
Applications Notes	This Human Methionine aminopeptidase 2 (METAP2) ELISA Kit employs a two-site sandwich ELISA to quantitate METAP2 in samples. An antibody specific for METAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMETAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for METAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of METAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703858	KTE61643	48 T	$339		Add to Cart
BHE13703858	KTE61643	96 T	$589		Add to Cart
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BHE13703858	KTE61643	96 Tx50	$24569		Add to Cart

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