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Home / ELISA Kit

Human Metallothionein-1F (MT1F) ELISA Kit

SKU : BHE13703728

MT1F (Metallothionein 1F) is a Protein Coding gene. Among its related pathways are Metabolism and Response to metal ions. An important paralog of this gene is MT1E. Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

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Mfr.No.	KTE61513
Description	This Human Metallothionein-1F (MT1F) ELISA Kit employs a two-site sandwich ELISA to quantitate MT1F.
Alternative Names	MT1F; MGC32732; MT1; metallothionein 1F (functional)
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Metallothionein-1F microplate Human Metallothionein-1F standard Human Metallothionein-1F detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Metallothionein-1F (MT1F) ELISA Kit has high sensitivity and excellent specificity for detection of Human MT1F. No significant cross-reactivity or interference between Human MT1F and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MT1F (Metallothionein 1F) is a Protein Coding gene. Among its related pathways are Metabolism and Response to metal ions. An important paralog of this gene is MT1E. Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Notes	This Human Metallothionein-1F (MT1F) ELISA Kit employs a two-site sandwich ELISA to quantitate MT1F in samples. An antibody specific for MT1F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT1F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT1F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT1F bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	4494
Antigen	MT1F
Tag	MT1F
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Metallothionein-1F (MT1F) ELISA Kit employs a two-site sandwich ELISA to quantitate MT1F in samples. An antibody specific for MT1F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT1F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT1F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT1F bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703728	KTE61513	48 T	$339		Add to Cart
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