Human Mesothelin(MSLN)ELISA KIT

Mfr.No.

CSB-E13382h

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Recovery

The recovery of bovine FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	94-108
EDTA plasma (n=4)	89	84-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-99
1:2	Average %	103
	Range %	97-107
1:4	Average %	91
	Range %	81-94
1:8	Average %	100
	Range %	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q13421
Tissue Specificity	Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

Applications	ELISA
Research Area	MSLN

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503335	CSB-E13382h	96T	$790	Add to Cart
BHE10503335	CSB-E13382h	5x96T	$3792	Add to Cart
BHE10503335	CSB-E13382h	10x96T	$7505	Add to Cart