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Home / ELISA Kit

Human Mesoderm induction early response protein 1 (MIER1) ELISA Kit

SKU : BHE13703834

MIER1 encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). Mesoderm induction early response protein 1 functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal.

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Mfr.No.	KTE61619
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mesoderm induction early response protein 1 (MIER1) ELISA Kit employs a two-site sandwich ELISA to quantitate MIER1.
Alternative Names	MIER1; DKFZp781G0451; ER1; KIAA1610; MGC131940; MGC150640; MGC150641; MI-ER1; RP5-944N15.1; hMI-ER1; mesoderm induction early response 1; mesoderm induction early response 1 (MI-ER1)
Detection Method	Colorimetric
Kit Components	Human Mesoderm induction early response protein 1 microplate Human Mesoderm induction early response protein 1 standard Human Mesoderm induction early response protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mesoderm induction early response protein 1 (MIER1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MIER1. No significant cross-reactivity or interference between Human MIER1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MIER1 encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). Mesoderm induction early response protein 1 functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal.
Notes	This Human Mesoderm induction early response protein 1 (MIER1) ELISA Kit employs a two-site sandwich ELISA to quantitate MIER1 in samples. An antibody specific for MIER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMIER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	57708

Applications	ELISA
Applications Notes	This Human Mesoderm induction early response protein 1 (MIER1) ELISA Kit employs a two-site sandwich ELISA to quantitate MIER1 in samples. An antibody specific for MIER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMIER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703834	KTE61619	48 T	$339		Add to Cart
BHE13703834	KTE61619	96 T	$589		Add to Cart
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