Human Meprin A Subunit Beta

Mfr.No.

E5663Hu

Sensitivity

0.027ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Endopeptidase-2;MEP1B;Meprin A subunit beta;Meprin B;N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta;PABA Peptide hydrolase;PPH beta

Molecular Weight

79571

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5663Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.61	0.11	4.28
Intra-Assay	2	18	8.29	0.33	4
Intra-Assay	3	18	4.19	0.27	6.54

Background

Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.

Protocol

These standard curves of E5663Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.346	2.238	2.196
	2.13
6ng/mL	1.345	1.293	1.251
	1.241
3ng/mL	0.794	0.803	0.761
	0.813
1.5ng/mL	0.5	0.483	0.441
	0.467
0.75ng/mL	0.231	0.211	0.168
	0.19
0ng/mL	0.036	0.042	0
	0.049

Gene ID	4225
UniProt ID	Q16820

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107025	E5663Hu	48T	$320		Add to Cart
BHE12107025	E5663Hu	96T	$458		Add to Cart

Human Meprin A Subunit Beta - MEP1B ELISA Kit

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