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Home / ELISA Kit

Human Membrane-associated progesterone receptor component 1(PGRMC1) ELISA kit

SKU : BHE10503327

This Human IL-35 ELISA Kit was designed for the quantitative measurement of Human IL-35 protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-EL017876HU

Sensitivity

11.7 pg/mL

Detection Range

47 pg/mL-3000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

progesterone receptor membrane component 1, HPR6.6, MPR, progesterone binding protein

Recovery

The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		92		87-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		94
		Range %		90-98
1:2		Average %		98
		Range %		94-103
1:4		Average %		97
		Range %		90-106
1:8		Average %		94
		Range %		88-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.872	2.752	2.812	2.705
2000	2.279	2.179	2.229	2.122
1000	1.545	1.589	1.567	1.460
500	0.952	1.002	0.977	0.870
250	0.531	0.575	0.553	0.446
125	0.341	0.366	0.354	0.247
62.5	0.255	0.275	0.265	0.158
0	0.108	0.105	0.107	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	O00264

Applications	ELISA
Research Area	PGRMC1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10503327	CSB-EL017876HU	96T	$695		Add to Cart
BHE10503327	CSB-EL017876HU	5x96T	$3336		Add to Cart
BHE10503327	CSB-EL017876HU	10x96T	$6602.50		Add to Cart

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