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Home / ELISA Kit

Human Melatonin receptor type 1B (MTNR1B) ELISA Kit

SKU : BHE13703698

MTNR1B encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. MTNR1B product is an integral membrane protein that is a G-protein coupled, 7-transmembrane receptor. It is found primarily in the retina and brain although this detection requires RT-PCR. It is thought to participate in light-dependent functions in the retina and may be involved in the neurobiological effects of melatonin.

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Mfr.No.	KTE61483
Description	This Human Melatonin receptor type 1B (MTNR1B) ELISA Kit employs a two-site sandwich ELISA to quantitate MTNR1B.
Alternative Names	MTNR1B; MEL-1B-R; MT2; melatonin receptor MEL1B; melatonin receptor type 1B
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Melatonin receptor type 1B microplate Human Melatonin receptor type 1B standard Human Melatonin receptor type 1B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Melatonin receptor type 1B (MTNR1B) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTNR1B. No significant cross-reactivity or interference between Human MTNR1B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MTNR1B encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. MTNR1B product is an integral membrane protein that is a G-protein coupled, 7-transmembrane receptor. It is found primarily in the retina and brain although this detection requires RT-PCR. It is thought to participate in light-dependent functions in the retina and may be involved in the neurobiological effects of melatonin.
Notes	This Human Melatonin receptor type 1B (MTNR1B) ELISA Kit employs a two-site sandwich ELISA to quantitate MTNR1B in samples. An antibody specific for MTNR1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTNR1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTNR1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTNR1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	4544
Antigen	MTNR1B
Tag	MTNR1B
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Melatonin receptor type 1B (MTNR1B) ELISA Kit employs a two-site sandwich ELISA to quantitate MTNR1B in samples. An antibody specific for MTNR1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTNR1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTNR1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTNR1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703698	KTE61483	48 T	$339		Add to Cart
BHE13703698	KTE61483	96 T	$589		Add to Cart
BHE13703698	KTE61483	96 Tx5	$2629		Add to Cart
BHE13703698	KTE61483	96 Tx50	$24569		Add to Cart

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