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Home / ELISA Kit

Human Melanotransferrin (MFI2) ELISA Kit

SKU : BHE13703853

Melanotransferrin encoded by MFI2 is a cell-surface glycoprotein found on melanoma cells. Melanotransferrin shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. MFI2 resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.

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Mfr.No.	KTE61638
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Melanotransferrin (MFI2) ELISA Kit employs a two-site sandwich ELISA to quantitate MFI2.
Alternative Names	MFI2; CD228; FLJ38863; MAP97; MGC4856; MTF1; melanoma-associated antigen p97; melanoma-associated antigen p97; isoform 2; melanotransferrin; membrane-bound transferrin-like protein
Detection Method	Colorimetric
Kit Components	Human Melanotransferrin microplate Human Melanotransferrin standard Human Melanotransferrin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Melanotransferrin (MFI2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MFI2. No significant cross-reactivity or interference between Human MFI2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Melanotransferrin encoded by MFI2 is a cell-surface glycoprotein found on melanoma cells. Melanotransferrin shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. MFI2 resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.
Notes	This Human Melanotransferrin (MFI2) ELISA Kit employs a two-site sandwich ELISA to quantitate MFI2 in samples. An antibody specific for MFI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMFI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFI2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4241

Applications	ELISA
Applications Notes	This Human Melanotransferrin (MFI2) ELISA Kit employs a two-site sandwich ELISA to quantitate MFI2 in samples. An antibody specific for MFI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMFI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFI2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703853	KTE61638	48 T	$339		Add to Cart
BHE13703853	KTE61638	96 T	$589		Add to Cart
BHE13703853	KTE61638	96 Tx5	$2629		Add to Cart
BHE13703853	KTE61638	96 Tx50	$24569		Add to Cart

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