Human Melanotransferrin - MFI2 ELISA Kit

Mfr.No.

E2491Hu

Sensitivity

0.024ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Melanotransferrin (also known as MFI2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD228;CD228;MAP97;Melanoma-associated antigen p97;Melanotransferrin;MFI2;MTf;MTF1

Molecular Weight

80215

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2491Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.85	0.15	4.01
Intra-Assay	2	18	6.38	0.18	2.78
Intra-Assay	3	18	2.99	0.13	4.48

Background

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.

Protocol

These standard curves of E2491Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.203	2.101	2.063
	1.999
6ng/mL	1.366	1.46	1.422
	1.554
3ng/mL	0.607	0.669	0.631
	0.731
1.5ng/mL	0.316	0.357	0.319
	0.397
0.75ng/mL	0.142	0.169	0.132
	0.197
0ng/mL	0.024	0.038	0
	0.052

Gene ID	4241
UniProt ID	P08582

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104029	E2491Hu	48T	$320		Add to Cart
BHE12104029	E2491Hu	96T	$458		Add to Cart

Human Melanotransferrin - MFI2 ELISA Kit

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